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KMID : 0380020080230050369
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 5 p.369 ~ p.374
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Asymmetric resolution of racemic styrene oxide using recombinant Escherichia coli harboring epoxide hydrolase of Rhodotorula glutinis
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Abstract
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The effects of reaction temperature and the addition of various detergents on the enantioselective hydrolysis activity of the recombinant Escherichia coli containing the epoxide hydrolase (EH) gene of Rhodotorula glutinis were investigated for the production of enantiopure styrene oxide. The recombinant E. coli harboring the EH gene from R. glutinis exhibited the enantiopreference toward (R)-styrene oxide with the maximum hydrolytic activity of 165.04 μmol/min/mg of dry cell weight (dcw). The addition of 0.5% (w/v) Tween 20 at 10℃ increased the initial hydrolysis rate and enantioselectivity by 1.45-fold and 2.0-fold, respectively. Enantiopure (S)-styrene oxide was prepared with 99% ee enantiopurity and 46.0% yield (theoretical yield=50%) from 20 mM racemic styrene oxide.
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KEYWORD
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enantioselective hydrolysis, epoxide hydrolase, Rhodotorula glutinis, enantiopure (S)-styrene oxide
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